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    <title>Affymetrix Hybridization Methods</title>  
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<a target="content" href="http://pubs.broadinstitute.org/mpr/projects/Leukemia/protocol_content.html#OV"><b> Overview</b></a><br>
<a target="content" href="http://pubs.broadinstitute.org/mpr/projects/Leukemia/protocol_content.html#FS"><b> First strand cDNA synthesis</b></a><br>
<a target="content" href="http://pubs.broadinstitute.org/mpr/projects/Leukemia/protocol_content.html#SS"><b> Second strand cDNA synthesis</b></a><br>
<a target="content" href="http://pubs.broadinstitute.org/mpr/projects/Leukemia/protocol_content.html#DSC"><b> Clean-up of dscDNA</b></a><br>
<a target="content" href="http://pubs.broadinstitute.org/mpr/projects/Leukemia/protocol_content.html#IVT"><b> In Vitro Transcription</b></a><br>
<a target="content" href="http://pubs.broadinstitute.org/mpr/projects/Leukemia/protocol_content.html#IC"><b> IVT Clean-up</b></a><br>
<a target="content" href="http://pubs.broadinstitute.org/mpr/projects/Leukemia/protocol_content.html#CRNA"><b> Fragmentation of cRNA</b></a><br>
<a target="content" href="http://pubs.broadinstitute.org/mpr/projects/Leukemia/protocol_content.html#AFFX"><b> Hybridization onto Chips</b></a><br>
<a target="content" href="http://pubs.broadinstitute.org/mpr/projects/Leukemia/protocol_content.html#AFFX"><b> Fluidics</b></a><br>
<a target="content" href="http://pubs.broadinstitute.org/mpr/projects/Leukemia/protocol_content.html#AFFX"><b> Pre-Scanning:  Creating *.exp files</b></a><br>
<a target="content" href="http://pubs.broadinstitute.org/mpr/projects/Leukemia/protocol_content.html#AFFX"><b> Washing &amp; Staining</b></a><br>
<a target="content" href="http://pubs.broadinstitute.org/mpr/projects/Leukemia/protocol_content.html#AFFX"><b> Scanning</b></a><br>
<a target="content" href="http://pubs.broadinstitute.org/mpr/projects/Leukemia/protocol_content.html#Appendix"><b>Appendix</b></a><br>


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